Titolo:  Metabolomics improves the histopathological diagnosis of asphyxial deaths: an animal proof-of-concept model
Data di pubblicazione:  2021
Autori:  Locci, E.; Chighine, A.; Noto, A.; Ferino, G.; Baldi, A.; Varvarousis, D.; Xanthos, T.; De-Giorgio, F.; Stocchero, M.; d'Aloja, E.
Numero degli autori:  10
Lingua:  Inglese
Presenza coautori internazionali: 
Volume:  11
Fascicolo:  1
Numero dell’articolo:  10102
Numero di pagine:  11
Digital Object Identifier (DOI):  http://dx.doi.org/10.1038/s41598-021-89570-0
Codice identificativo Pubmed:  33980966
Codice identificativo Scopus:  2-s2.0-85105786315
Codice identificativo ISI:  WOS:000652602700011
URL:  https://www.nature.com/articles/s41598-021-89570-0
Abstract:  The diagnosis of mechanical asphyxia remains one of the most difficult issues in forensic pathology. Asphyxia ultimately results in cardiac arrest (CA) and, as there are no specific markers, the differential diagnosis of primitive CA and CA secondary to asphyxiation relies on circumstantial details and on the pathologist experience, lacking objective evidence. Histological examination is currently considered the gold standard for CA post-mortem diagnosis. Here we present the comparative results of histopathology versus those previously obtained by 1H nuclear magnetic resonance (NMR) metabolomics in a swine model, originally designed for clinical purposes, exposed to two different CA causes, namely ventricular fibrillation and asphyxia. While heart and brain microscopical analysis could identify the damage induced by CA without providing any additional information on the CA cause, metabolomics allowed the identification of clearly different profiles between the two groups and showed major differences between asphyxiated animals with good and poor outcomes. Minute-by-minute plasma sampling allowed to associate these modifications to the pre-arrest asphyxial phase showing a clear correlation to the cellular effect of mechanical asphyxia reproduced in the experiment. The results suggest that metabolomics provides additional evidence beyond that obtained by histology and immunohistochemistry in the differential diagnosis of CA.
Parole chiave:  metabolomics; asphyxial death; histopathological diagnosis
Tipologia: 1.1 Articolo in rivista

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