Titolo:  On the role of a coumarin derivative for sensing applications: Nucleotide identification using a micellar system
Data di pubblicazione:  2016
Autori:  Bettoschi, Alexandre; Ceglie, Andrea; Lopez, Francesco; Meli, Valeria; Murgia, Sergio; Tamburro, Manuela; Caltagirone, Claudia; Cuomo, Francesca
Numero degli autori:  8
Lingua:  Inglese
Presenza coautori internazionali:  no
Volume:  477
Pagina iniziale:  8
Pagina finale:  15
Numero di pagine:  8
Digital Object Identifier (DOI):  http://dx.doi.org/10.1016/j.jcis.2016.05.034
Codice identificativo Pubmed:  27236839
Codice identificativo Scopus:  2-s2.0-84971516815
Codice identificativo ISI:  WOS:000379451900002
Abstract:  The recognition of nucleotides is of crucial importance because they are the basic constituents of nucleic acids. The present study is focused on the selective interaction between a novel amphiphilic fluorophore containing coumarin and imidazole, CI (1-methyl-3-(12-((2-oxo-2H-chromen-7-yl)oxy)dodecyl)-1H-imi dazol-3-ium bromide), and different nucleotide-monophosphates (NMPs). It was supposed that the solubilization of the low water soluble CI in a micelle system of hexadecyltrimethylammonium chloride (CTAC) would make the coumarin moiety of CI available to the interaction with the water-soluble NMPs. Changes in CTAC critical micelle concentration suggested that CI strongly interacted with the host cationic surfactant, thus forming a positively charged interface enriched with coumarin able to interact with the anionic NMPs. Steady-state fluorescence quenching revealed that CI/CTAC system was capable of distinguish between purine- and pyrimidine-based nucleotides. A modified Stern-Volmer equation permitted the use of a quenching model that accounted for the possible interactions between the micelles and the nucleotides. The data analysis allowed calculating selective parameters that differentiated according to the type of nucleotide either at 25 or 50 °C. Our results established the utility of the novel coumarin derivative fluorophore, supported by the simple and suitable micellar systems, as a tool for DNA sensing applications.
Parole chiave:  Coumarin; Fluorescence quenching; Micelles; Nucleotides; Surfaces, coatings and films; Electronic, optical and magnetic materials; Biomaterials; Colloid and surface chemistry
Tipologia: 1.1 Articolo in rivista

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