FA/0209 - BIOSENSORS
Academic Year 2022/2023
Free text for the University
STEFANO LAI (Tit.)
- Teaching style
- Lingua Insegnamento
|[60/76] BIOTECHNOLOGY||[76/20 - Ord. 2018] Farmaceutico||4||32|
The course will provide students a general view about technologies and applications of sensors for chemical and biological species.
-Acquiring knowledge and understanding: the student will learn fundamental concepts, technologies and issues related to the development of biochemical sensors, including golden standard systems and new generation devices;
-Appying knowledge and understanding: the student will be able to apply the foreground on biochemical sensors and systems for the setup of laboratory experiments.
-Making informed judgements and choices: the student will be able to understand data and plots related to biochemical sensors, evaluating performances and, if necessary, defining strategies for performance improvement and optimization;
- Communicating knowledge and understanding: the student will be able to report about the foreground using appropriate technical terms, and to use appropriate metrics to describe performances of considered applications:
- Capacities to continue learning: the student will be able to integrate the concepts related to different fields – physics, chemistry, biology and electronics – for the analysis of biochemical sensors; the student will be also able to integrate the concepts provided during lessons with the most up-to-date results reported in literature.
Basic concept of physics, physic of the solid matter and chemistry are fundamental prerequisites.
- INTRODUCTION TO THE COURSE (1h)
- INTRODUCTION TO BIOCHEMICAL SENSORS (5h): sensor definition and main figures of merits. Direct vs. indirect detection, label vs. label-free approaches. Biochemical sensors as case study: main blocks. Paradigms for the development of biochemical sensors.
- ANALYTES AND BIORECEPTORS (5h): main properties of biomolecules of interest for the development of biosensors; main techniques for the realization of the biochemical receptor layer (physisorption, chemical functionalization, entrapment of biomolecules);
- BIOCHEMICAL SENSORS: organized according to transduction principle:
o Optical sensors (6h): introduction to optical properties of matter, fluorescence, FRET and quenching. State-of-art applications (immunofluorescence, ELISA, DNA microarray, PCR and real time PCR). New concepts for optical biosensors: Lab-on-Chip and SPR.
o Electrochemical sensors (10h): basics on electrical devices and components; basics on electrochemistry (redox reactions, electrochemical cells, electrochemical principles and models). State-of-the-art about electrochemical sensors: potentiometric sensors based on ion-selective membranes, enzymatic sensors (potentiometric, first- and second-generation amperometric, conductometric). Novel approaches to biochemical electrochemical sensors: third-generation enzymatic sensors, amperometric immunosensors, voltammetric and impedimetric techniques and their application to biochemical sensing.
o Electronic sensors (5h): introduction to transistors: technologies and basic working principles. From MOSFET to ISFET for pH detection. From ISFET to BioFET for the detection of biochemical reactions. New technologies for electronic biosensors.
Students will acquire the main concepts of the course by attending to frontal lessons.
Verification of learning
The final mark will be established by means of written tests, including open questions and multiple-choice questions. The maximum score will be 32/30: with a score equal or larger than 31, the student will receive the magna cum laude
-Slides of the lessons;
-De Rossi, Ahluwalia, Mazzoldi, Pede, Scilingo, “Sensori per misure biomediche”, Collana di Ingegneria Biomedica, Patron Editore.
Basic concept provided in the course will be integrated with the most up-to-date publications in the field of bioelectronics.