FA/0103 - BIOINGEGNERIA AMBIENTALE CON LABORATORIO
Academic Year 2019/2020
Free text for the University
GIORGIA DE GIOANNIS (Tit.)
- Teaching style
- Lingua Insegnamento
|[60/56] INDUSTRIAL BIOTECHNOLOGY||[60/56-00 - Ord. 2016] PERCORSO COMUNE||6||60|
At the end of the course the student will have acquired specific knowledge on the growth systems of microorganisms, both from the experimental (through specific tests in the Laboratory) and the theoretical point of views.
In particular, the student will know the main parameters through which he describes, measures and orients the bacterial growth, as well as know the main mathematical equations used for the kinetic study of microbial growth and the conduction of a conventional biological reactor.
Furthermore, some modern technologies will be described in which biological systems are used for the production of bioproducts and bioenergy even from waste substrates.
No specific pre-requisites are mandatory. However, in order to profitably follow the Course, it is appropriate to have knowledge of General Chemistry, Analytical Chemistry, Organic Chemistry, Biochemistry and General Microbiology.
FIRST PART (THEORY)
Biorefineries: definitions and future. The environmental problem in biotechnology.
Recalls of mathematical analysis.
Kinetics and stoichiometry in the growth of microorganisms (growth phases, order of the reaction, kinetics of Monod, determination of kinetic constants with Lineweaver-Burk).
Reactor. Definitions and equations of operation of the BATCH, CSTR and PLUG FLOW reactors.
SECOND PART (LAB).
Safety elements in the laboratory. Notions of safety, structure and management of security, fire prevention. Safety in a chemical laboratory. Reactivity and flammability of chemical compounds. Chemical risk: labeling, symbology and risk phrases; dose-response, acute and chronic toxicity, monitoring of exposure and effects. Electric risk. Analytical parameters and basic scientific instrumentation. Total solids, volatile (suspended and dissolved), solid sedimentable, COD, BOD; Main basic analytical tools: use and calibration. Start-up of a lab-scale experiment Preparation of acidic, basic and buffer solutions; Preparation of equipment; Preparation of the microbial culture for the inoculation of the bioreactors; Preparation of the growth substrate. Determination of biological activity of a biomass. Batch determination of the kinetic constants of heterotrophic bacteria.
24 hours theory
36 hours lab
Verification of learning
The student evaluation includes an oral exam in which 3 questions are proposed on the theoretical part and 2 on the laboratory part. To pass the exam, the student must demonstrate that he possesses the basic theoretical and practical knowledge for the study and application of industrial and environmental bioengineering processes.
The score of the exam is assigned by a vote expressed in thirtieths.
The final evaluation of the student will be obtained as the average of the grade obtained for the theoretical and laboratory modules.