Title:  Irreversible affinity immobilization of lentil seedling amine oxidase with activity retention
Internal authors: 
Issue Date:  2007
Journal: 
ENVIRONMENTAL ENGINEERING AND MANAGEMENT JOURNAL  
Abstract:  An aliphatic-amine-bearing chromatographic support, based on commercial silica, was prepared and tested as potential matrix for affinity immobilization of a copper/topaquinone-containing amine oxidase from lentil seedlings. The support proved to be noticeably effective in immobilizing the enzyme with concomitant purification and activity retention. The immobilization yields and some properties of this preparation were also determined and compared with those of the soluble enzyme. High activity retention, good operational stability and low preparative costs are best combined in this promising immobilized amine oxidase.
URI:  http://hdl.handle.net/11584/27236
Type: 1.1 Articolo in rivista

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