Assembly of Multicomponent Nano-Bioconjugates Composed of Mesoporous Silica Nanoparticles, Proteins, and Gold Nanoparticles

DELPIANO, GIULIA ROSSELLA;Casula, Maria F.;Piludu, Marco;Corpino, Riccardo;Ricci, Pier Carlo;Vallet-Regí, María;Sanjust, Enrico;Monduzzi, Maura;Salis, Andrea
Last
2019

Abstract

The purpose of this work was the assembly of multicomponent nano-bioconjugates based on mesoporous silica nanoparticles (MSNs), proteins (bovine serum albumin, BSA, or lysozyme, LYZ), and gold nanoparticles (GNPs). These nano-bioconjugates may find applications in nanomedicine as theranostic devices. Indeed, MSNs can act as drug carriers, proteins stabilize MSNs within the bloodstream, or may have therapeutic or targeting functions. Finally, GNPs can either be used as contrast agents for imaging or for photothermal therapy. Here, amino-functionalized MSNs (MSN–NH2) were synthesized and characterized through various techniques (small angle X-rays scattering TEM, N2 adsorption/desorption isotherms, and thermogravimetric analysis (TGA)). BSA or lysozyme were then grafted on the external surface of MSN–NH2 to obtain MSN–BSA and MSN–LYZ bioconjugates, respectively. Protein immobilization on MSNs surface was confirmed by Fourier transform infrared spectroscopy, ζ-potential measurements, and TGA, which also allowed the estimation of protein loading. The MSN–protein samples were then dispersed in a GNP solution to obtain MSN–protein–GNPs nano-bioconjugates. Transmission electron microscopy (TEM) analysis showed the occurrence of GNPs on the MSN–protein surface, whereas almost no GNPs occurred in the protein-free control samples. Fluorescence and Raman spectroscopies suggested that proteins–GNP interactions involve tryptophan residues.
Inglese
4
6
11044
11052
9
https://pubs.acs.org/doi/full/10.1021/acsomega.9b01240
Esperti anonimi
internazionale
scientifica
Delpiano, GIULIA ROSSELLA; Casula, Maria F.; Piludu, Marco; Corpino, Riccardo; Ricci, Pier Carlo; Vallet-Regí, María; Sanjust, Enrico; Monduzzi, Maura; Salis, Andrea
1.1 Articolo in rivista
info:eu-repo/semantics/article
1 Contributo su Rivista::1.1 Articolo in rivista
262
9
open
Files in This Item:
File Size Format  
2019 ACS Omega Delpiano et al.pdf

open access

Description: articolo principale
Type: versione editoriale
Size 9.06 MB
Format Adobe PDF
9.06 MB Adobe PDF View/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Questionnaire and social

Share on:
Impostazioni cookie