||Azoxystrobin, kresoxim-methyl, trifloxystrobin, pyraclostrobin, famoxadone and fenamidone are permitted Qo Inhibitor (QoI) fungicides applied to vine in some European countries for the treatment of downy and powdery mildews. In this work, a method is validated for the analysis of these fungicides in grapes and wine. This screening method consists in a simple one step liquid-liquid extraction followed by liquid chromatography (LC) fitted with a diode array detector (DAD). Limits of detection for grapes and wine were below 0.2 mg kg-1 or mg l-1, precision was not above 13%, and recoveries were, on average, 95 ± 5% for grapes and 104 ± 6% for wine. Global uncertainties evaluated in the concentration range from 0.25 to 2.50 mg l-1 were below 20%. A confirmatory method by gas chromatography (GC) with mass spectrometry (MS) detection was used.