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Professor
GIORGIA DE GIOANNIS (Tit.)
FABIANO ASUNIS
Period
First Semester 
Teaching style
Convenzionale 
Lingua Insegnamento
ITALIANO 



Informazioni aggiuntive

Course Curriculum CFU Length(h)
[60/76]  BIOTECHNOLOGY [76/10 - Ord. 2018]  Industriale e Ambientale 12 120

Objectives

The module ReBio aims to provide students with knowledge related to biological reactors. In particular, the student will know the main parameters through which bacterial growth is described, measured and oriented, as well as will know the main basic mathematical equations used for the kinetic study of microbial growth and the management of a conventional biological reactor. In addition, some modern technologies in which biological reactors are used, especially in the environmental field, will be described.
The student will acquire the ability to understand the main problems posed both by the choice of the type and by the operation of the conventional biological reactors used in environmental field. The module aims both to motivate the student to work alone and to promote teamwork.
The module BioRaf aims to provide students with the knowledge relating to the main production processes of products of interest for industrial and environmental biotechnology, which can be obtained by applying the concept of biorefinery.
At the end of the course the student will have acquired the knowledge: i) of the matrices to be processed, including waste, ii) of the main production processes, with particular attention to the fermentation processes, and iii) of the main downstream processes for the recovery of the products obtained .
The planned activities of the module will allow the student to know and understand the operations necessary for the characterization of a matrix, preparation and management of tests in lab scale reactors in which to carry out experiments for the production of biomethane, biohydrogen and other products of interest from waste (liquid and solid). He will also become familiar with the equipment in a chemical-environmental laboratory.


The course aims both to motivate the student to work alone and to promote teamwork. The student will be able to process and critically interpret the results obtained from instrumental analyzes and laboratory-scale experiments conducted. The student will also be able to define, even quantitatively, hypothetical biotechnological chains in which to process waste feedstocks by applying the knowledge and skills acquired, outlining the general design criteria according to principles of environmental and economic sustainability.

Prerequisites

There are no pre-established preparations, however, in order to successfully follow the Integrated Course, knowledge of General Chemistry, Analytical Chemistry, Organic Chemistry, Biochemistry and General Microbiology is appropriate.

Contents

ReBio:
Part A) Introduction to the course. Review of mathematical analysis. (6 h frontal lesson)
Part B) Kinetics and stoichiometry in the growth of microorganisms (growth stages, reaction order, Monod kinetics, determination of kinetic constants). (16 h frontal lesson + 8 h seminars in the laboratory)
Part C) Reactors. Definitions and working equations of the biological reactors BATCH, SBR, CSTR and PLUG FLOW. Main applications and uses of biological reactors (10 h frontal lesson + 8 h laboratory seminars)


BioRaf
Part A) Introduction to the course. Main analytical parameters and basic scientific instrumentation. Analysis of total, volatile (suspended and dissolved) solids content, COD and BOD measurements. (2 h seminar + 8 h laboratory). The concept of biorefinery. Main feedstocks and characterization. Biodegradable residues and valorisation prospects. The case of waste: Waste biorefineries. (5 h seminar + 15 h laboratory)
Part B) Production of energy and biofuels from waste biomass: Start-up of a lab-scale experiment for the production of Biogas, Biohydrogen, Bioethanol and biodiesel, Bioelectricity. Determination of the anaerobic biological activity of a bacterial inoculum. (5 h seminar + 15 h laboratory)
Part C) Production of materials: Start-up of a lab-scale experiment for the production of bioplastics, chemicals with high added value. (5 h seminar + 15 h laboratory). Downstream processes (2 h seminar).

Teaching Methods

Rebio
Frontal lesson: 32 hours
Seminars in the laboratory: 16 hours

BioRaf
Laboratory: 53 h
Seminars: 19 h

Verification of learning

ReBio
The student's assessment includes an oral test in which 3 questions are proposed, one for each part of the module (A, B and C). To pass the module exam, the student must demonstrate that he or she has at least sufficient knowledge in all questions. The exam test score is attributed by a mark expressed in thirtieths.
The final assessment of the student relative to the module will be obtained as a weighted average of the mark obtained for the single questions asked, according to the weight attributed by the teacher to the single question.

BioRaf
The student's assessment will be based on 3 individual reports of max 6000 words each on laboratory experiences (one for each part of the module (A, B and C) and each evaluated with a score from 1 to 10) and an oral test in which 3 questions are proposed, one for each part of the module (A, B and C). To pass the exam for the module, the student must demonstrate to have at least sufficient knowledge in all oral questions and at least sufficient evaluation in all individual reports presented. The exam test score is attributed by a mark expressed in thirtieths. The final assessment of the student relative to the module will be obtained as a weighted average of the mark obtained for the single questions asked, according to the weight attributed by the teacher to the single question.

Texts

Lecture notes and slides provided by the teachers. Open access scientific articles provided by the teachers

More Information

The ReBio module contributes to the achievement of the following Sustainable development goals:

6. Clean Water and Sanitation
7. Affordable and Clean Energy
9. Industry, Innovation, and Infrastructure

The BioRaf module contributes to the achievement of the following Sustainable development goals:
6. Clean Water and Sanitation
7. Affordable and Clean Energy
9. Industry, Innovation, and Infrastructure
12. Responsible Consumption and Production
13. Climate Action
14. Life Below Water
15. Life On Land

Questionnaire and social

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